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pcDNA3 HA-VPS4A E228Q
(Plasmid #246736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246736 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS4A E228Q (DN)
  • Species
    H. sapiens (human)
  • Mutation
    E228Q
  • Entrez Gene
    VPS4A (a.k.a. CIMDAG, SKD1, SKD1A, SKD2, VPS4, VPS4-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.18.638932 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA-VPS4A E228Q was a gift from Yihong Ye (Addgene plasmid # 246736 ; http://n2t.net/addgene:246736 ; RRID:Addgene_246736)

  • For your References section:

    CHIP protects lysosomes from CLN4 mutant-induced membrane damage. Lee J, Chin N, Zou J, Mazli WNAB, Jarnik M, Saidi L, Xu Y, Jeong E, Suh J, Replogle J, Ward ME, Bonifacino JS, Zheng W, Hao L, Ye Y. Nat Cell Biol. 2025 Aug 25. doi: 10.1038/s41556-025-01738-2. 10.1038/s41556-025-01738-2 PubMed 40855364
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