CHIP protects lysosomes from CLN4 mutant-induced membrane damage.
Lee J, Chin N, Zou J, Mazli WNAB, Jarnik M, Saidi L, Xu Y, Jeong E, Suh J, Replogle J, Ward ME, Bonifacino JS, Zheng W, Hao L, Ye Y
Nat Cell Biol. 2025 Aug 25. doi: 10.1038/s41556-025-01738-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246722 | FSW CHIP delTPR-Myc | Neuron-specific expression of human CHIP with TPR domain deleted, and a myc-tag on its C-terminus |
| 246723 | FSW CHIP delU-box-Myc | Neuron-specific expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus |
| 246724 | FSW CHIP-Myc | Neuron-specific expression of human CHIP wildtype, and a myc-tag on its C-terminus |
| 246725 | FSW FLAG | Neuron-specific expression of insert with a FLAG tag on its N-terminus after cloning |
| 246726 | FSW mKeima | Neuron-specific expression of insert with a mKeima tag on its N-terminus after cloning |
| 246727 | FSW mKeima-DNAJC5 | Neuron-specific expression of human DNAJC5 wild type, and a mKeima tag on its N-terminus |
| 246728 | FSW mKeima-DNAJC5 delL116 | Neuron-specific expression of human DNAJC5 with L116 deleted, and a mKeima tag on its N-terminus |
| 246729 | FSW TMEM192-3xHA | Neuron-specific expression of human TMEM192 with a tag of 3x HA on its C-terminus |
| 246730 | mCerulean-DNAJC5 | Mammalian expression of human DNAJC5 wildtype with a mCerulean tag on its N-terminus |
| 246731 | mCerulean-DNAJC5 delL116 | Mammalian expression of human DNAJC5 L116 deleted with a mCerulean tag on its N-terminus |
| 246732 | mCerulean-DNAJC5 L115R | Mammalian expression of human DNAJC5 L115R with a mCerulean tag on its N-terminus |
| 246733 | mCitrine-DNAJC5 | Mammalian expression of human DNAJC5 wildtype with a mCitrine (YFP) tag on its N-terminus |
| 246734 | mCitrine-DNAJC5 delL116 | Mammalian expression of human DNAJC5 L116 deleted with a mCitrine (YFP) tag on its N-terminus |
| 246735 | mCitrine-DNAJC5 L115R | Mammalian expression of human DNAJC5 L115R with a mCitrine (YFP) tag on its N-terminus |
| 246736 | pcDNA3 HA-VPS4A E228Q | Mammalian expression of human VPS4A E228Q (dominant negative form) with a HA tag on its N-terminus |
| 246737 | pCMV6 CHIP delTPR-Myc | Mammalian expression of human CHIP with TPR domain deleted, and a myc-tag on its C-terminus |
| 246738 | pCMV6 CHIP delU-box-Myc | Mammalian expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus |
| 246739 | pCMV6 CHIP-Myc | Mammalian expression of human CHIP wildtype, and a myc-tag on its C-terminus |
| 246740 | pCMV6 FLAG-DNAJC5 | Mammalian expression of human DNAJC5 with L116 deleted, and a FLAG tag on its N-terminus |
| 246741 | pCMV6 FLAG-DNAJC5 delL116 | Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus |
| 246742 | pCMV6 FLAG-DNAJC5 L115R | Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus |
| 246743 | pGEX GST-tev-hDNAJC5 | Bacterial expression of human DNAJC5 wildtype with GST and a cleavage site for TEV protease on its N-terminus |
| 246744 | pGEX GST-tev-hDNAJC5 delL116 | Bacterial expression of human DNAJC5 L116 deleted with GST and a cleavage site for TEV protease on its N-terminus |
| 246745 | pGEX GST-tev-hDNAJC5 L115R | Bacterial expression of human DNAJC5 L115R with GST and a cleavage site for TEV protease on its N-terminus |
| 246746 | pLenti mKeima | Mammalian and lentiviral expression of insert with a mKeima tag on its N-terminus |
| 246747 | pLenti mKeima-DNAJC5 | Mammalian and lentiviral expression of human DNAJC5 wildtype with a mKeima tag on its N-terminus |
| 246748 | pLenti mKeima-DNAJC5 delJ | Mammalian and lentiviral expression of human DNAJC5 J domain deleted with a mKeima tag on its N-terminus |
| 246749 | pLenti mKeima-DNAJC5 delL116 | Mammalian and lentiviral expression of human DNAJC5 L116 deleted with a mKeima tag on its N-terminus |
| 246750 | pLenti mKeima-DNAJC5 L115R | Mammalian and lentiviral expression of human DNAJC5 L115R with a mKeima tag on its N-terminus |
| 246751 | sh-hCHIP | Mammalian and lentiviral expression of shRNA targeting human CHIP (Stub1) |
| 246752 | pLKO.1/CFP sh-hUBE2N | Mammalian and lentiviral expression of shRNA targeting human UBE2N (Ubc13) |