AiP16391 - pAAV-AiE0450h-minBG-DDC(GENSAT)-3xHA-WPRE3-bGHpA (Alias: HCT120)
(Plasmid
#246882)
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PurposeDesigned to drive AAV-mediated DDC-HA expression in striatal MSNs in presence of GENSAT intron
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Depositing Lab
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Depositing OrganizationAllen Institute
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonerAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDC
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SpeciesH. sapiens (human)
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Entrez GeneDDC (a.k.a. AADC)
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Tag
/ Fusion Protein
- 3X HA (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.20.700641 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AiP16391 - pAAV-AiE0450h-minBG-DDC(GENSAT)-3xHA-WPRE3-bGHpA (Alias: HCT120) was a gift from Tanya Daigle (Addgene plasmid # 246882 ; http://n2t.net/addgene:246882 ; RRID:Addgene_246882) -
For your References section:
Cell type-selective AADC expression reduces motor deficits and dyskinesia in Parkinson’s disease mouse models. Chowdhury A, Fraser A, Departee M, Taskin N, Quinlan MA, Mich JK, Omstead V, Lerma N, Opitz-Araya X, Hughes AC, Kussick E, Martinez R, Reding M, Liang E, Shulga L, Rette D, Huang C, Casian B, Leibly M, Helback O, Barcelli T, Wood T, Uribe N, Bacon C, Bowlus J, Newman D, Kutsal R, Khem S, Donadio N, Yao S, Ronellenfitch K, Wright V, Gudsnuk K, Horwitz GD, Levi BP, Lein ES, Ting JT, Daigle TL. bioRxiv 2026.01.20.700641 10.64898/2026.01.20.700641