|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24689||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameInsulin-like growth factor 2
SpeciesX. laevis (frog)
Insert Size (bp)478
Entrez Geneigf2-b (a.k.a. igf2, IGF-II, insigf, pp9974, MGC80113)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For sense linearize with NotI, transcribe with SP6.
For antisense, linearize with EcoRI, transcribe with T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xIGF2 was a gift from Edward De Robertis (Addgene plasmid # 24689 ; http://n2t.net/addgene:24689 ; RRID:Addgene_24689)
For your References section:Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer. Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, De Robertis EM. Mech Dev. 2002 Aug . 116(1-2):169-72. 10.1016/S0925-4773(02)00123-5 PubMed 12128218
Map uploaded by the depositor.