|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12248||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10242
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
Gene/Insert nameshRNA against GFP
MutationNGFRdel is in place of GFP on the backbone.
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLVTH-siGFP was a gift from Didier Trono (Addgene plasmid # 12248 ; http://n2t.net/addgene:12248 ; RRID:Addgene_12248)
For your References section:Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M, Trono D. J Virol. 2003 Aug . 77(16):8957-61. 10.1128/JVI.77.16.8957-8951.2003 PubMed 12885912