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PASTA-1_pUC19_HDR-Bxb1-attG_TRAC_for-Cas12a
(Plasmid #247037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247037 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 3333
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bxb1 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
  • Species
    H. sapiens (human); Mycolicibacterium smegmatis

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This HDR-template encoding plasmid can be used together with eeBxb1 mRNA, which can be generated by IVT from David Liu’s Addgene plasmid #222339 ( https://www.addgene.org/222339/ ). It enables targeted, (ee)Bxb1-mediated insertion of our integration-plasmid ‘PASTA-2_trunc-pUC19_INT-Bxb1-attV_P2A_CD19CAR_bGH-pA’ (Addgene #247038, provided alongside) into the human TRAC gene. Please visit https://doi.org/10.1101/2025.09.10.675267 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PASTA-1_pUC19_HDR-Bxb1-attG_TRAC_for-Cas12a was a gift from Dimitrios Wagner (Addgene plasmid # 247037 ; http://n2t.net/addgene:247037 ; RRID:Addgene_247037)

  • For your References section:

    Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases. Kassing I, Kath J, Nitulescu A-M, Glaser V, Hartmann LM, Pu Y, Huth L, Kārkliņš R, Shaji S, Ringel AR, Pouzolles M, Stein M, Ibrahim DM, Wagner DL. bioRxiv 2025.09.10.675267 10.1101/2025.09.10.675267
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