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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(F294A, Y311A)
(Plasmid #247132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247132 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone size w/o insert (bp) 5413
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Argonaute2
  • Alt name
    AGO2
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2958
  • Mutation
    F294A, Y311A
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG-SUMO(Eu1) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(F294A, Y311A) was a gift from David Bartel (Addgene plasmid # 247132 ; http://n2t.net/addgene:247132 ; RRID:Addgene_247132)

  • For your References section:

    The E3 ubiquitin ligase mechanism specifying targeted microRNA degradation. Farnung J, Slobodyanyuk E, Wang PY, Blodgett LW, Lin DH, von Gronau S, Schulman BA, Bartel DP. Nature. 2026 Apr;652(8110):784-793. doi: 10.1038/s41586-026-10232-0. Epub 2026 Mar 18. 10.1038/s41586-026-10232-0 PubMed 41851464
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