The E3 ubiquitin ligase mechanism specifying targeted microRNA degradation.
Farnung J, Slobodyanyuk E, Wang PY, Blodgett LW, Lin DH, von Gronau S, Schulman BA, Bartel DP
Nature. 2026 Apr;652(8110):784-793. doi: 10.1038/s41586-026-10232-0. Epub 2026 Mar 18.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247112 | pLJM_3xHA_HsAGO2 | Expression of AGO to assay in-cell microRNA stability |
| 247113 | pLJM_3xHA_HsAGO2(F294A) | Expression of AGO to assay in-cell microRNA stability |
| 247114 | pLJM_3xHA_HsAGO2(F294A, Y311A) | Expression of AGO to assay in-cell microRNA stability |
| 247115 | pLJM_3xHA_HsAGO2(R315V, H316A) | Expression of AGO to assay in-cell microRNA stability |
| 247116 | pLJM_3xHA_HsAGO2(d296-305) | Expression of AGO to assay in-cell microRNA stability |
| 247117 | pLJM_3xHA_HsAGO2(d332-336) | Expression of AGO to assay in-cell microRNA stability |
| 247118 | pLJM_3xHA_HsAGO2(K335A, H336A, T337A) | Expression of AGO to assay in-cell microRNA stability |
| 247119 | pLJM_3xHA_HsAGO2(F491A) | Expression of AGO to assay in-cell microRNA stability |
| 247120 | pLJM_3xHA_HsAGO2(K493A) | Expression of AGO to assay in-cell microRNA stability |
| 247121 | pLJM_3xHA_HsAGO2(M504A) | Expression of AGO to assay in-cell microRNA stability |
| 247122 | pLJM_3xHA_HsAGO2(F491A, K493A, M504A, H507A) | Expression of AGO to assay in-cell microRNA stability |
| 247123 | pLJM_3xHA_HsAGO2(E464A, V465A, K468A) | Expression of AGO to assay in-cell microRNA stability |
| 247124 | pLJM_3xHA_HsAGO2(R479A) | Expression of AGO to assay in-cell microRNA stability |
| 247125 | pLJM_3xHA_HsAGO2(Q486A, Q488A) | Expression of AGO to assay in-cell microRNA stability |
| 247126 | pLJM_3xHA_HsAGO2(d81_85) | Expression of AGO to assay in-cell microRNA stability |
| 247127 | pLJM_3xHA_HsAGO2(E116A + d118_127) | Expression of AGO to assay in-cell microRNA stability |
| 247128 | pLJM_3xHA_HsAGO2(H77A, H81A, T118A, P120A) | Expression of AGO to assay in-cell microRNA stability |
| 247129 | pLJM_3xHA_HsAGO2(KRclusters5+6) | Expression of AGO to assay in-cell microRNA stability |
| 247130 | pLJM_3xHA_HsAGO2(KRx40) | Expression of AGO to assay in-cell microRNA stability |
| 247131 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(F294A) | Expression of AGO for protein purification |
| 247132 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(F294A, Y311A) | Expression of AGO for protein purification |
| 247134 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(d296-305) | Expression of AGO for protein purification |
| 247135 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(d332-336) | Expression of AGO for protein purification |
| 247136 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(K335A, H336A, T337A) | Expression of AGO for protein purification |
| 247137 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO1 | Expression of AGO for protein purification |
| 254347 | pDARMO_CMVTv2_3xFLAG-SUMO_Eu1-HsZSWIM8-3xHA | Expression of ZSWIM8 for protein purification |
| 254348 | pRK5_HsELOB | Expression of ELOB for protein purification |
| 254349 | pRK5_HsELOC | Expression of ELOC for protein purification |
| 254350 | piggyBac_Tet-On_HsZSWIM8 | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254351 | piggyBac_Tet-On_HsZSWIM8(H199A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254352 | piggyBac_Tet-On_HsZSWIM8(P75A, L76A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254353 | piggyBac_Tet-On_HsZSWIM8(V100A, P101A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254354 | piggyBac_Tet-On_HsZSWIM8(mono) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254355 | piggyBac_Tet-On_HsZSWIM8(Δdimer) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254356 | piggyBac_Tet-On_HsZSWIM8(E91R, E94R) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254357 | piggyBac_Tet-On_HsZSWIM8(VHL-box) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254358 | piggyBac_Tet-On_HsZSWIM8(Δ311-325) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254359 | piggyBac_Tet-On_HsZSWIM8(312-316A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254360 | piggyBac_Tet-On_HsZSWIM8(Y320A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254361 | piggyBac_Tet-On_HsZSWIM8(337-343A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254362 | piggyBac_Tet-On_HsZSWIM8(L1372A, N1373A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254363 | piggyBac_Tet-On_HsZSWIM8(T1303A, Y1304A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254364 | piggyBac_Tet-On_HsZSWIM8(F1261A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254365 | piggyBac_Tet-On_HsZSWIM8(R1345A, R1348A, R1350A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254366 | piggyBac_Tet-On_HsZSWIM8(R1345E, R1348E, R1350E) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254367 | piggyBac_Tet-On_HsZSWIM8(S1341A, D1344A, R1345A, R1348A, R1350A, N1375A, R1379A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254368 | piggyBac_Tet-On_HsZSWIM8(T1303A, Y1304A, S1341A, D1344A, R1345A, R1348A, R1350A, N1375A, R1379A) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254369 | piggyBac_Tet-On_HsZSWIM8(H395E, H401E, R404E) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254370 | piggyBac_Tet-On_HsZSWIM8(K460E, R461E, H464E, K465E, K466E, R470E) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254371 | piggyBac_Tet-On_HsZSWIM8(K808E, K815E, K817E, K818E, K820E, R825E) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254372 | piggyBac_Tet-On_HsZSWIM8(H395E, H401E, R404E, K460E, R461E, H464E, K465E, K466E, R470E, K808E, K815E, K817E, K818E, K820E,R825E) | Expression of ZSWIM8 to assay in-cell microRNA stability |
| 254373 | piggyBac_Tet-On_BFP-3xHA | Expression of BFP as a negative control in the ZSWIM8 in-cell microRNA stability assay |
| 254374 | pET28a_6xHis-thrombin-SUMO-HsTNRC6B(599-683)-3xHA | Expression of TNRC6B fragment for protein purification |
| 254375 | pET28a_6xHis-thrombin-SUMO-HsTNRC6B(599-683)-3xFLAG | Expression of TNRC6B fragment for protein purification |
| 254376 | pcDNA3.3_3xFLAG-SUMO_Eu1-HsAGO2(F491A) | Expression of AGO for protein purification |
| 254377 | pcDNA3.3_3xFLAG-SUMO_Eu1-HsAGO2(K493A) | Expression of AGO for protein purification |