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pET28a_6xHis-thrombin-SUMO-HsTNRC6B(599-683)-3xFLAG
(Plasmid #254375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254375 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5239
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human TNRC6B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    699
  • Mutation
    Fragment consisting of residues 599-683
  • Entrez Gene
    TNRC6B (a.k.a. GDSBA)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis-thrombin-SUMO (N terminal on insert)
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_6xHis-thrombin-SUMO-HsTNRC6B(599-683)-3xFLAG was a gift from David Bartel (Addgene plasmid # 254375 ; http://n2t.net/addgene:254375 ; RRID:Addgene_254375)

  • For your References section:

    The E3 ubiquitin ligase mechanism specifying targeted microRNA degradation. Farnung J, Slobodyanyuk E, Wang PY, Blodgett LW, Lin DH, von Gronau S, Schulman BA, Bartel DP. Nature. 2026 Apr;652(8110):784-793. doi: 10.1038/s41586-026-10232-0. Epub 2026 Mar 18. 10.1038/s41586-026-10232-0 PubMed 41851464
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