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piggyBac_Tet-On_BFP-3xHA
(Plasmid #254373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254373 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    piggyBac_Tet-on
  • Backbone size w/o insert (bp) 7511
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EBFP2
  • Species
    Synthetic
  • Insert Size (bp)
    834
  • Promoter TRE3G
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggyBac_Tet-On_BFP-3xHA was a gift from David Bartel (Addgene plasmid # 254373 ; http://n2t.net/addgene:254373 ; RRID:Addgene_254373)

  • For your References section:

    The E3 ubiquitin ligase mechanism specifying targeted microRNA degradation. Farnung J, Slobodyanyuk E, Wang PY, Blodgett LW, Lin DH, von Gronau S, Schulman BA, Bartel DP. Nature. 2026 Apr;652(8110):784-793. doi: 10.1038/s41586-026-10232-0. Epub 2026 Mar 18. 10.1038/s41586-026-10232-0 PubMed 41851464
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