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pCMV-Sport6 Baf60b
(Plasmid #24723)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24723 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-Sport6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4396
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Baf60b
  • Alt name
    Smarcd2
  • Alt name
    Bruneau Lab Plasmid 203
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Smarcd2 (a.k.a. Baf60b, AW322457)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer SP6, CMV-F
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In situ probe: Cut with EcoRV or KpnI, use T7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Sport6 Baf60b was a gift from Benoit Bruneau (Addgene plasmid # 24723 ; http://n2t.net/addgene:24723 ; RRID:Addgene_24723)
  • For your References section:

    Baf60c is essential for function of BAF chromatin remodelling complexes in heart development. Lickert H, Takeuchi JK, Von Both I, Walls JR, McAuliffe F, Adamson SL, Henkelman RM, Wrana JL, Rossant J, Bruneau BG. Nature. 2004 Nov 4. 432(7013):107-12. 10.1038/nature03071 PubMed 15525990