pcDNA3 CMV Cyclone-EGFP
(Plasmid
#247495)
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PurposeAcyclovir-regulated EGFP
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Depositing Lab
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Depositing OrganizationCornell University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 247495-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 247495-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3-EGFP (Plasmid #13031)
- Total vector size (bp) 6935
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Modifications to backboneNone
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP gene with Cyclone insertion
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Alt nameCyclone-EGFP
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SpeciesSynthetic
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Insert Size (bp)1496
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MutationCyclone was inserted after Glutamine94 of EGFP to gain acyclovir control.
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 247495-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 247495-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 CMV Cyclone-EGFP was a gift from Samie Jaffrey (Addgene plasmid # 247495 ; http://n2t.net/addgene:247495 ; RRID:Addgene_247495) -
For your References section:
A portable poison exon for small-molecule control of mammalian gene expression. Hou Q, Oleynikov M, Mei X, Dong L, Hagen T, Jaffrey SR. Nat Methods. 2025 Nov 3. doi: 10.1038/s41592-025-02860-7. 10.1038/s41592-025-02860-7 PubMed 41184552
Map uploaded by the depositor.
