pNICBio2_USP7:207-573
(Plasmid #247540)

• Purpose: E. coli expression for protein production. May not contain entire coding region of gene.
• Depositing Lab: Cheryl Arrowsmith
• Depositing Organization: University of Toronto
  Located in Canada
• Publication: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion (unpublished)

Backbone

• Vector backbone: pNICBio2
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: USP7
• Species: H. sapiens (human)
• Mutation: none
• Entrez Gene: USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
• Promoter: T7
• Tags / Fusion Proteins:
  • MHHHHHHHHHHDLGTENLYFQSM (N terminal on insert)
  • SSKGGYGLNDIFEAQKIEWHE (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: T7 term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: NM_003470

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.08.669300 for bioRxiv preprint.