p28BIOH-LIC_USP7:520-1067
(Plasmid
#247541)
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PurposeE. coli expression for protein production. May not contain entire coding region of gene.
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Depositing Lab
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Depositing OrganizationUniversity of Toronto
Located in Canada -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247541 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonep28BIOH-LIC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUSP7
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SpeciesH. sapiens (human)
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Mutationnone
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Entrez GeneUSP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
- Promoter T7
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Tags
/ Fusion Proteins
- MSGLNDIFEAQKIEWHEGSAGGSG (N terminal on insert)
- GGSGHHHHHH (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer T7 term
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byNM_003470
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.08.08.669300 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p28BIOH-LIC_USP7:520-1067 was a gift from Cheryl Arrowsmith (Addgene plasmid # 247541 ; http://n2t.net/addgene:247541 ; RRID:Addgene_247541)
