pLEX307-miRFP680
(Plasmid
#247684)
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Purpose(Empty Backbone) Lentivirus compatible Gateway destination vector. For constitutive expression under the EF1alpha promoter with constitutive expression of miRFP680.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247684 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLEX307
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Backbone manufacturerDavid Root (Addgene #41392)
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Vector typeMammalian Expression, Lentiviral, Gateway Cloning
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Selectable markersmiRFP680
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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A portion of this plasmid was derived from a plasmid made byVladislav Verkhusha (miRFP680 is from Addgene #136557)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-miRFP680 was a gift from Sho Iketani (Addgene plasmid # 247684 ; http://n2t.net/addgene:247684 ; RRID:Addgene_247684) -
For your References section:
Cross-resistance patterns in SARS-CoV-2 against 3CL protease inhibitors. Chithelen J, Lovett DH, Wang K, Torres Yanagisawa AE, Caccin FNM, Iketani S. Nat Commun. 2026 May 19;17(1):6575. doi: 10.1038/s41467-026-73444-y. 10.1038/s41467-026-73444-y PubMed 42156379