pDONOR-tagBFP-PSM-dTOMATO-GBA1
(Plasmid
#247979)
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PurposeHDR donor vector to revert GBA1 N370S to WT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDONOR-tagBFP-PSM-dTOMATO
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Backbone manufacturerJens Schwamborn, Addgene plasmid #100604
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Vector typeHDR Donor plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGBA1 Exon 10 to Exon 12
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SpeciesH. sapiens (human)
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Entrez GeneGBA1 (a.k.a. GBA, GCB, GLUC)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.23.661126 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONOR-tagBFP-PSM-dTOMATO-GBA1 was a gift from Ellen Sidransky (Addgene plasmid # 247979 ; http://n2t.net/addgene:247979 ; RRID:Addgene_247979) -
For your References section:
Bidirectional regulation of glycoprotein nonmetastatic melanoma protein B by beta-glucocerebrosidase deficiency in GBA1 isogenic dopaminergic neurons from a patient with Gaucher disease and parkinsonism. Chen C, Ma C, Sam R, Lichtenberg J, Chen T, Hao Y, Li Z, Kowal I, Andersh K, Qi YA, Perez G, Hertz E, Li Y, Williams D, Henderson MJ, Park M, Jiang X, Jerez PA, Blauwendraat C, Sidransky E, Chen Y. bioRxiv [Preprint]. 2025 Jun 25:2025.06.23.661126. doi: 10.1101/2025.06.23.661126. 10.1101/2025.06.23.661126 PubMed 40667145