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ptGSVG
(Plasmid #248056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248056 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pK7WG2
  • Backbone size w/o insert (bp) 8151
  • Vector type
    Plant Expression
  • Selectable markers
    Neomycin (select with G418) ; Oleosin1-GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arabidopsis thaliana RPS5A promoter
  • Alt name
    Plastid presequence of A. thaliana RecA1; A. thaliana WHY2; cytidine deaminase domain of B. cenocepacia deaminase toxin
  • Alt name
    uracil glycosylase inhibitor of Bacillus phage AR9; CaMV 35S terminator; Oleosin1-GFP fusion
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    6999
  • Mutation
    4 amino acid changes (S1326G, G1348S, A1398V, S1418G) in cytidine deaminase domain, lowers deaminase activity

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.01.685978 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptGSVG was a gift from Shin-ichi Arimura (Addgene plasmid # 248056 ; http://n2t.net/addgene:248056 ; RRID:Addgene_248056)

  • For your References section:

    Chloroplast- and Mitochondrion-Specific Random C-to-T Mutagenesis for Forward Genetics of Organelle Genomes. Kosaka N, Harada Y, Nakazato I, Okuno M, Itoh T, Tsutsumi N, Arimura SI. bioRxiv 2025.11.01.685978 10.1101/2025.11.01.685978
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