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T7-5'UTR-bpNLS-BxbI-3'UTR-polyA (CJT476)
(Plasmid #248216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248216 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    IVT-nCas9(H840A)
  • Backbone manufacturer
    Addgene #207455
  • Vector type
    Mammalian Expression, CRISPR ; in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5'UTR-bpNLS-BxbI-3'UTR-polyA
  • Alt name
    CJT476
  • Species
    Synthetic
  • Mutation
    None
  • Promoter T7
  • Tag / Fusion Protein
    • BPNLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-5'UTR-bpNLS-BxbI-3'UTR-polyA (CJT476) was a gift from Benjamin Kleinstiver (Addgene plasmid # 248216 ; http://n2t.net/addgene:248216 ; RRID:Addgene_248216)

  • For your References section:

    Immune evasive DNA donors and recombinases license kilobase-scale writing. Tou CJ, Xie K, Ferreira da Silva J, Kalailingam P, Amar-Lewis E, Rufino-Ramos D, Sawyer W, Eller ML, Starzyk J, Majumdar I, Wang J, Lee D, Yang S, Meis RJ, Dahl GA, Li J, Shan R, Artzi N, Musolino PL, Wu H, Kleinstiver BP. Nature. 2026 Mar 11. doi: 10.1038/s41586-026-10241-z. 10.1038/s41586-026-10241-z PubMed 41813887
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