Immune evasive DNA donors and recombinases license kilobase-scale writing.
Tou CJ, Xie K, Ferreira da Silva J, Kalailingam P, Amar-Lewis E, Rufino-Ramos D, Sawyer W, Eller ML, Starzyk J, Majumdar I, Wang J, Lee D, Yang S, Meis RJ, Dahl GA, Li J, Shan R, Artzi N, Musolino PL, Wu H, Kleinstiver BP
Nature. 2026 Mar 11. doi: 10.1038/s41586-026-10241-z.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248208 | pCMV-bpNLS-Bxb1 (EMK569) | Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter. |
| 248209 | pCMV-bpNLS-eeBxb1 (CJT555) | eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter. |
| 248210 | pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604) | SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter. |
| 248211 | pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605) | bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter. |
| 248212 | pCMV-bpNLS-Stab-exin21-eeBxb1-Stab (CJT607) | bpNLS-, stabilon-, and exin21-tagged eeBxb1 recombinase, expressed from a CMV promoter. |
| 248213 | pCMV-bpNLS-IS621 (CJT489) | bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter. |
| 248214 | pCMV-Piv (CJT504) | Piv invertase, expressed from a CMV promoter. |
| 248215 | pCMV-ePiv (CJT553) | ePiv invertase, expressed from a CMV promoter. |
| 248216 | T7-5'UTR-bpNLS-BxbI-3'UTR-polyA (CJT476) | bpNLS-BxbI IVT vector for mRNA production |
| 248217 | T7-5'UTR-bpNLS-BceINT-3'UTR-polyA (CJT483) | bpNLS-BceINT IVT vector for mRNA production |
| 248219 | T7-5'UTR-bpNLS-SacINT-3'UTR-polyA (CJT485) | bpNLS-SacINT IVT vector for mRNA production |
| 248220 | T7-5'UTR-SuperDn29-bpNLS-3'UTR-polyA (CJT611) | bpNLS-SuperDn29 IVT vector for mRNA production |
| 248221 | T7-5'UTR-IS621-NLS-3'UTR-polyA (CJT503) | IS621-bpNLS IVT vector for mRNA production |
| 248222 | T7-5'UTR-PE7-3'UTR-polyA (CJT612) | PE7 IVT vector for mRNA production |
| 248223 | Bxb1 attB pDonor (JO1529) | Bxb1 attB pDonor with Nt.BbvcI site for cssDNA production |
| 248224 | Bxb1 attP pDonor (CJT453) | Bxb1 attP pDonor with Nt.BbvcI site for cssDNA production |
| 248225 | Pa01 attP60 pDonor (CJT460) | Pa01 attP pDonor with Nt.BbvcI site for cssDNA production |
| 248226 | BceINT attP pDonor (CJT486) | BceINT attP pDonor with Nt.BbvcI site for cssDNA production |
| 248227 | SacINT attP pDonor (CJT487) | SacINT attP pDonor with Nt.BbvcI site for cssDNA production |
| 248228 | Dn29 attD pDonor (CJT454) | Dn29 attP pDonor with Nt.BbvcI site for cssDNA production |
| 248231 | LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR (DRR724) | Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attB target sites |
| 248232 | LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728) | Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites |