Immune evasive DNA donors and recombinases license kilobase-scale writing.
Tou CJ, Xie K, Ferreira da Silva J, Kalailingam P, Amar-Lewis E, Rufino-Ramos D, Sawyer W, Eller ML, Starzyk J, Majumdar I, Wang J, Lee D, Yang S, Meis RJ, Dahl GA, Li J, Shan R, Artzi N, Musolino PL, Wu H, Kleinstiver BP
Nature. 2026 Mar 11. doi: 10.1038/s41586-026-10241-z.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248208 | pCMV-bpNLS-Bxb1 (EMK569) | Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter. |
| 248209 | pCMV-bpNLS-eeBxb1 (CJT555) | eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter. |
| 248210 | pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604) | SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter. |
| 248211 | pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605) | bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter. |
| 248213 | pCMV-bpNLS-IS621 (CJT489) | bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter. |
| 248215 | pCMV-ePiv (CJT553) | ePiv invertase, expressed from a CMV promoter. |
| 248231 | LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR (DRR724) | Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attB target sites |
| 248232 | LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728) | Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites |