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pCAG-VPS13C-delVABdelATG2C-3xFLAG
(Plasmid #248320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248320 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS13C
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of residues 3477-3584, replaced by GS; Deletion of residues 2412-3067, replaced by 3x(GGGGS)
  • Entrez Gene
    VPS13C (a.k.a. BLTP5C, PARK23)
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.10.687702 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-VPS13C-delVABdelATG2C-3xFLAG was a gift from Karin Reinisch (Addgene plasmid # 248320 ; http://n2t.net/addgene:248320 ; RRID:Addgene_248320)

  • For your References section:

    Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin. Li D, Wang X, Hao H, Eden J, Hu B, Walsh EE, Parson MAH, Hamill S, Li Y, Chen G, Burke JE, De Camilli P, Reinisch KM. Mol Cell. 2026 Jul 7:S1097-2765(26)00417-X. doi: 10.1016/j.molcel.2026.06.028. 10.1016/j.molcel.2026.06.028 PubMed 42413490
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