Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin.
Li D, Wang X, Hao H, Eden J, Hu B, Walsh EE, Parson MAH, Hamill S, Li Y, Chen G, Burke JE, De Camilli P, Reinisch KM
Mol Cell. 2026 Jul 7:S1097-2765(26)00417-X. doi: 10.1016/j.molcel.2026.06.028.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248315 | pCAG-VPS13C-WT-3xFLAG | Mammalian expression of VPS13C for in vitro studies |
| 248316 | pCAG-VPS13C-delATG2C-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248317 | pCAG-VPS13C-delC-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248318 | pCAG-VPS13C-delVAB-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248319 | pCAG-VPS13C-delCdelWWE-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248320 | pCAG-VPS13C-delVABdelATG2C-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248321 | pCAG-VPS13C-VAB-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248322 | pCMV10-VPS13C-WT-GFP-3xFLAG | Mammalian expression of VPS13C for in vitro studies |
| 248323 | pCMV10-VPS13C-M392A W393D W395K I398D-GFP-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248324 | pCMV10-VPS13C-W374K I378D L382K-GFP-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248325 | pCMV10-VPS13C-W374K W393D W395K-GFP-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248326 | pCMV10-VPS13C-W395C-GFP-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248327 | pCMV10-VPS13C-delC-GFP-3xFLAG | Mammalian expression of VPS13C mutant for in vitro studies |
| 248328 | pCAG-Strep-Rab7-Q67L | Mammalian expression of Rab7 mutant for in vitro studies |
| 248329 | pCAG-MBP-TEV-Rab7-Q67L-6His | Mammalian expression of Rab7 mutant for in vitro studies |
| 248330 | pCAG- 3xFLAG-CtVPS13 | Mammalian expression of VPS13 from C. Thermophilum for in vitro studies |
| 248331 | pETDuet-6xHis-Strep-CtVPS13 (1-635) | Bacterial expression of a N-temrinal fragment of VPS13 from C. Thermophilum for in vitro studies |
| 248332 | pETDuet-6xHis-Strep-CtVPS13 (1-635) + 6xHis-HsCaM | Bacterial expression of a N-temrinal fragment of VPS13 from C. Thermophilum with human calmodulin for in vitro studies |
| 255527 | pET29-Rab7(Q67L)-6xHis | Bacterial expression of Rab7A mutants for in vitro studies |
| 255528 | pET29-Rab7(T22N)-6xHis | Bacterial expression of Rab7A mutants for in vitro studies |
| 255693 | pCMV-VPS13C(1-1390)-mStayGold | N-terminal fragment of VPS13C with mStayGold tag |
| 255695 | pPB-CAG-VPS13C^mStayGold | Full length VPS13C with internal mStayGold tag |