Skip to main content
Addgene
Open Menu

pETDuet-6xHis-Strep-CtVPS13 (1-635) + 6xHis-HsCaM
(Plasmid #248332)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248332 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pETDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    VPS13
  • Species
    Chaetomium thermophilum
  • Mutation
    Contains residues 1-635 only
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Calmodulin
  • Alt name
    HsCaM
  • Species
    H. sapiens (human)
  • Entrez Gene
    CALM1 (a.k.a. CALML2, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CPVT4, DD132, LQT14, PHKD, PHKD1, caM)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.10.687702 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-6xHis-Strep-CtVPS13 (1-635) + 6xHis-HsCaM was a gift from Karin Reinisch (Addgene plasmid # 248332 ; http://n2t.net/addgene:248332 ; RRID:Addgene_248332)

  • For your References section:

    Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin. Li D, Wang X, Hao H, Eden J, Hu B, Walsh EE, Parson MAH, Hamill S, Li Y, Chen G, Burke JE, De Camilli P, Reinisch KM. Mol Cell. 2026 Jul 7:S1097-2765(26)00417-X. doi: 10.1016/j.molcel.2026.06.028. 10.1016/j.molcel.2026.06.028 PubMed 42413490
Related items: