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pET29-Rab7(T22N)-6xHis
(Plasmid #255528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255528 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET29
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RAB7A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    621
  • Mutation
    changed Threonine 22 to Asparagine
  • Entrez Gene
    RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • Hexahistidine (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.10.687702 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29-Rab7(T22N)-6xHis was a gift from Karin Reinisch (Addgene plasmid # 255528 ; http://n2t.net/addgene:255528 ; RRID:Addgene_255528)

  • For your References section:

    Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin. Li D, Wang X, Hao H, Eden J, Hu B, Walsh EE, Parson MAH, Hamill S, Li Y, Chen G, Burke JE, De Camilli P, Reinisch KM. Mol Cell. 2026 Jul 7:S1097-2765(26)00417-X. doi: 10.1016/j.molcel.2026.06.028. 10.1016/j.molcel.2026.06.028 PubMed 42413490
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