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human p53-Δ(325-355)
(Plasmid #24871)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24871 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1089
  • Mutation
    Deletion of aa 325-355
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E.Coli strain for epression: BL21DE3-pLysS

p53 proteins are expressed better and more stable at low temperature

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human p53-Δ(325-355) was a gift from Cheryl Arrowsmith (Addgene plasmid # 24871 ; http://n2t.net/addgene:24871 ; RRID:Addgene_24871)
  • For your References section:

    Latent and active p53 are identical in conformation. Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH. Nat Struct Biol. 2001 Sep . 8(9):756-60. 10.1038/nsb0901-756 PubMed 11524676