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Gal4-HA-HIF1α(530-652)
(Plasmid #24887)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24887 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5252
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha or similar
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hypoxia inducible factor1alpha
  • Alt name
    Gal4-HA-HIF-ODD
  • Alt name
    oxygen-dependent degradation domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    909
  • Mutation
    partial HIF1alpha cDNA (encoding aa 530-652)fused to Gal4 DNA binding domain, fused to HA-tag
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Tags / Fusion Proteins
    • Gal4dbd (N terminal on backbone)
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer CMV-forw or T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal4-HA-HIF1α(530-652) was a gift from William Kaelin (Addgene plasmid # 24887 ; http://n2t.net/addgene:24887 ; RRID:Addgene_24887)
  • For your References section:

    HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Ivan M, Kondo K, Yang H, Kim W, Valiando J, Ohh M, Salic A, Asara JM, Lane WS, Kaelin WG. Science. 2001 Apr 20. 292(5516):464-8. 10.1126/science.1059817 PubMed 11292862