plenti-ALFA-SpdCas9-2xNLS
(Plasmid
#248898)
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PurposeLentiviral vector expressing human codon-optimized, ALFA-tagged SpdCas9 (deactivated DNAse domain)
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneLenti_Cas9_2A_Blast
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Backbone manufacturerJason Moffat (Addgene #73310)
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5xALFA-FLAG-SpdCas9-2xNLS-BlastR
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SpeciesStreptococcus pyogenes
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MutationD10A/H840A
- Promoter eIFa
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti-ALFA-SpdCas9-2xNLS was a gift from Michael Aregger (Addgene plasmid # 248898 ; http://n2t.net/addgene:248898 ; RRID:Addgene_248898)
