Michael Aregger Lab: An Optimized Cas12a Toolkit for Scalable Combinatorial Genetic Screening and Single-Cell Transcriptomics
Aregger M.
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248896 | pLenti-D156R LbCas12a-8xNLS | Lentiviral vector expressing human codon-optimized D156R LbCas12a with 8xNLS |
| 248897 | pLenti-hyper LbCas12a-8xNLS | Lentiviral vector expressing human codon-optimized hyper LbCas12a with 8xNLS |
| 248898 | plenti-ALFA-SpdCas9-2xNLS | Lentiviral vector expressing human codon-optimized, ALFA-tagged SpdCas9 (deactivated DNAse domain) |
| 248899 | pLenti-D156R LbdCas12a-8xNLS-ALFA | Lentiviral vector expressing human codon-optimized, ALFA-tagged D156R LbdCas12a (deactivated DNAse domain) |
| 248900 | pLenti-hyper LbdCas12a-8xNLS-ALFA | Lentiviral vector expressing human codon-optimized, ALFA-tagged hyper LbdCas12a (deactivated DNAse domain) |
| 248901 | plenti-ZIM3-SpdCas9-2xNLS | Lentiviral vector expressing human codon-optimized SpdCas9 (deactivated DNAse domain) fused to ZIM3 KRAB domain |
| 248902 | pLenti-D156R LbdCas12a-8xNLS-ZIM3 | Lentiviral vector expressing human codon-optimized D156R LbdCas12a (deactivated DNAse domain) fused to ZIM3 KRAB domain |
| 248903 | pLenti-hyper LbdCas12a-8xNLS-ZIM3 | Lentiviral vector expressing human codon-optimized hyper LbdCas12a (deactivated DNAse domain) fused to ZIM3 KRAB domain |
| 248904 | pLCHKOv6 | Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker |
| 248905 | pLCHKOv6_ZIM3-NbALFA | Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker, alongside hpGK driven ZIM3 KRAB domain (CRISPRi) fused to an ALFA nanobody. |
| 248906 | pLCHKOv6_miniVPR-NbALFA | Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker, alongside hpGK driven miniVPR (CRISPRa) fused to an ALFA nanobody. |
| 248907 | pLCHKOv6_miniVPR-NbALFA_tetO-EGFP | Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker, alongside hpGK driven miniVPR fused to ALFA nanobody, and tetO EGFP reporter. |