pLCHKOv6_ZIM3-NbALFA
(Plasmid #248905)

• Purpose: (Empty Backbone) Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker, alongside hpGK driven ZIM3 KRAB domain (CRISPRi) fused to an ALFA nanobody.
• Depositing Lab: Michael Aregger
• Depositing Organization: National Cancer Institute (NCI)
  Located in the United States of America
• Publication: An Optimized Cas12a Toolkit for Scalable Combinatorial Genetic Screening and Single-Cell Transcriptomics (unpublished)

Backbone

• Vector backbone: pLCHKOv6
• Backbone manufacturer: Michael Aregger (Addgene #248904)
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • Myc (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • Myc (C terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mikko Taipale (ZIM3 was sourced from Addgene #154472) and Motoyuki Hattori (NbALFA was sourced from Addgene #159986; codon-optimized sequence was synthesized).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HA-ZIM3-NbALFA-4xNLS-PuroR