pLenti-D156R LbdCas12a-8xNLS-ZIM3
(Plasmid
#248902)
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PurposeLentiviral vector expressing human codon-optimized D156R LbdCas12a (deactivated DNAse domain) fused to ZIM3 KRAB domain
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLenti-D156R LbCas12a-8xNLS
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Backbone manufacturerMichael Aregger (Addgene #248896)
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameD156R-LbdCas12a-8xNLS-ZIM3-Myc-NeoR
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SpeciesLachnospiraceae bacterium
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MutationD156R/D832R
- Promoter eIFa
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unspecified
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMikko Taipale (ZIM3 was sourced from Addgene #154472).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-D156R LbdCas12a-8xNLS-ZIM3 was a gift from Michael Aregger (Addgene plasmid # 248902 ; http://n2t.net/addgene:248902 ; RRID:Addgene_248902)