pLCHKOv6_miniVPR-NbALFA
(Plasmid
#248906)
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Purpose(Empty Backbone) Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker, alongside hpGK driven miniVPR (CRISPRa) fused to an ALFA nanobody.
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLCHKOv6
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Backbone manufacturerMichael Aregger (Addgene #248904)
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- Myc (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- Myc (C terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byStanley Qi (miniVPR was sourced from Addgene #183955) and Motoyuki Hattori (NbALFA was sourced from Addgene #159986; codon-optimized sequence was synthesized).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HA-miniVPR-NbALFA-4xNLS-PuroR
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLCHKOv6_miniVPR-NbALFA was a gift from Michael Aregger (Addgene plasmid # 248906 ; http://n2t.net/addgene:248906 ; RRID:Addgene_248906)
