plenti-ZIM3-SpdCas9-2xNLS
(Plasmid #248901)

• Purpose: Lentiviral vector expressing human codon-optimized SpdCas9 (deactivated DNAse domain) fused to ZIM3 KRAB domain
• Depositing Lab: Michael Aregger
• Depositing Organization: National Cancer Institute (NCI)
  Located in the United States of America
• Publication: An Optimized Cas12a Toolkit for Scalable Combinatorial Genetic Screening and Single-Cell Transcriptomics (unpublished)

Backbone

• Vector backbone: Lenti_Cas9_2A_Blast
• Backbone manufacturer: Jason Moffat (Addgene #73310)
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZIM3-FLAG-SpdCas9-2xNLS-BlastR
• Species: Streptococcus pyogenes
• Mutation: D10A/H840A
• Promoter: eIFa
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mikko Taipale (ZIM3 was sourced from Addgene #154472).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry