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pLenti-hyper LbdCas12a-8xNLS-ZIM3
(Plasmid #248903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248903 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLenti-hyper LbCas12a-8xNLS
  • Backbone manufacturer
    Michael Aregger (Addgene #248897)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hyper-LbdCas12a-8xNLS-ZIM3-Myc-NeoR
  • Species
    Lachnospiraceae bacterium
  • Mutation
    D156R/D235R/E292R/D350R/D832R
  • Promoter eIFa
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mikko Taipale (ZIM3 was sourced from Addgene #154472).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-hyper LbdCas12a-8xNLS-ZIM3 was a gift from Michael Aregger (Addgene plasmid # 248903 ; http://n2t.net/addgene:248903 ; RRID:Addgene_248903)
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