pTet_PflHamMA(deltaA)B
(Plasmid
#248938)
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PurposeHachiman from P. fluorescens under pTet control with the dHamA domain removed
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonep15a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePflHamMA(deltaA)B
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SpeciesPseudomonas fluorescens
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MutationHamA domain deletion
Cloning Information
- Cloning method Other
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTet_PflHamMA(deltaA)B was a gift from Jennifer Doudna (Addgene plasmid # 248938 ; http://n2t.net/addgene:248938 ; RRID:Addgene_248938) -
For your References section:
Recurrent acquisition of nuclease-protease pairs in antiviral immunity. Tuck OT, Hu JJ, Lopez SC, Adler BA, O'Brien CE, Hsieh K, Meredith C, Loi KJ, Yoon PH, Doherty EE, Lahiri A, Doudna JA. Science. 2026 Jan 8;391(6781):195-201. doi: 10.1126/science.aea8769. Epub 2025 Nov 13. 10.1126/science.aea8769 PubMed 41231971