Recurrent acquisition of nuclease-protease pairs in antiviral immunity.
Tuck OT, Hu JJ, Lopez SC, Adler BA, O'Brien CE, Hsieh K, Meredith C, Loi KJ, Yoon PH, Doherty EE, Lahiri A, Doudna JA
Science. 2026 Jan 8;391(6781):195-201. doi: 10.1126/science.aea8769. Epub 2025 Nov 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248931 | pTet_PflHamMAB | Hachiman from P. fluorescens under pTet control |
| 248933 | pTet_PflHamMA(S157A)B | Hachiman from P. fluorescens under pTet control with HamA mutated (S157A) |
| 248934 | pTet_PflHamMAB(D207A) | Hachiman from P. fluorescens under pTet control with HamB mutated (D207A) |
| 248935 | pTet_PflHamM(I118K)AB | Hachiman from P. fluorescens under pTet control with HamM mutated (I118K) |
| 248937 | pTet_PflHamM(I118K,I218K)AB | Hachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K) |
| 248938 | pTet_PflHamMA(deltaA)B | Hachiman from P. fluorescens under pTet control with the dHamA domain removed |
| 248939 | pTet_PflHamMA(S157A)(deltaA)B | Hachiman from P. fluorescens under pTet control with the dHamA domain removed and with a protease S157A mutation |
| 248940 | pTet_PflHamMA(-cJun)B | Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker |
| 248941 | pTet_PflHamMA(S157A)(-cJun)B | Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker and the protease mutated (S157A) |
| 248942 | pTet_PflHamM(pre-cut)AB | Hachiman from P. fluorescens under pTet control with HamM programmed breaks in the peptide backbone at I118 and I218 |
| 248943 | exp_PflHamM | His-MBP-HamM from P. fluorescens for overexpression. |
| 248945 | pLG017_Avs1a(I134K) | AVAST from E. piriflorinigrans with Avs1a mutated (I134K) |
| 248946 | pLG017_Avs1a(I234K) | AVAST from E. piriflorinigrans with Avs1a mutated (I234K) |
| 248947 | pLG017_Avs1a(I134K,I234K) | AVAST from E. piriflorinigrans with Avs1a mutated (I134K, I234K) |
| 248948 | pLG017_FLAG-Avs1a | AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a |
| 248949 | pLG017_FLAG-Avs1a(I134K,I234K) | AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K) |
| 248950 | pLG017_Avs1a_chimera | AVAST from E. piriflorinigrans with Avs1a replaced with Avs1a from Citrobacter Avs1a |
| 248951 | pJEX_P1terminase | phage P1 large terminase subunit under control of a crystal violet inducible promoter |
| 248952 | pJEX_lambdaterminase | phage lambda large terminase subunit under control of a crystal violet inducible promoter |
| 248953 | pTet_KaeCanABC | CanABC from K. aerogenes under control of pTet and the native promoter |
| 248954 | pTet_KaeCanA(C187A)BC | CanABC from K. aerogenes under control of pTet and the native promoter, with CanA mutated (C187A) |
| 248955 | pTet_KaeCanABC(H271A) | CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (H271A) |
| 248956 | pTet_KaeCanABC(G104K) | CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G104K) |
| 248957 | pTet_KaeCanABC(G189K) | CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G189K) |
| 248958 | pTet_KaeCanABC(G104K,G189K) | CanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K) |
| 248959 | exp_KaeCanAB | His-MBP-CanAB from K. aerogenes for overexpression. |
| 248960 | exp_KaeCanC | His-MBP-CanC from K. aerogenes for overexpression. |