pDEST/FRT/TO/3xFLAG-SCAF1-SCAF4CID
(Plasmid
#249043)
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Purposefor expression of N-terminally 3xFLAG tagged SCAF1 transcript containing CID domain from SCAF4 instead of SRI, in human cells
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Depositing Lab
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Depositing OrganizationUniversity of Copenhagen
Located in Denmark -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDEST/TO/3xFLAG
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSCAF1
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SpeciesH. sapiens (human)
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MutationSubstitution of the SRI domain sequence with the CID domain of SCAF4
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Entrez GeneSCAF1 (a.k.a. SRA1)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2025.12.08.691990 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST/FRT/TO/3xFLAG-SCAF1-SCAF4CID was a gift from Lea Gregersen (Addgene plasmid # 249043 ; http://n2t.net/addgene:249043 ; RRID:Addgene_249043)
