Lea Gregersen Lab: SCAF1 driven polyadenylation site usage regulates mRNA isoform expression and neuronal differentiation
Kompocholi S, Stamidis N, Liu H, Hjortø GM, Kafkia E, Ruidiaz SF, Miller TCR, Żylicz JJ, Gregersen LH
bioRxiv 2025.12.08.691990
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249038 | pENTR4_SCAF1 | for expression of SCAF1 transcript in human cells |
| 249039 | pENTR4_SCAF1-ΔSRI | for expression of SCAF1 transcript lacking the SRI domain, in human cells |
| 249040 | pDEST/FLAGHA/TO-SCAF1 | for expression of SCAF1 transcript N-terminally tagged with FLAGHA |
| 249041 | pDEST/GFP/TO-SCAF1 | for expression of SCAF1 transcript N-terminally tagged with GFP |
| 249043 | pDEST/FRT/TO/3xFLAG-SCAF1-SCAF4CID | for expression of N-terminally 3xFLAG tagged SCAF1 transcript containing CID domain from SCAF4 instead of SRI, in human cells |
| 249044 | pHisSUMO-SCAF1SRI | His tagged SRI domain of SCAF1 expressed together with His-SUMO, for domain purification (codon optimized sequence of SRI domain) |
| 249045 | SCAF1-mAID-FLAG degron plasmid | for endogenously auxin-degron cassete tagging SCAF1 gene locus in mouse cells (C-terminus insertion) |