pHisSUMO-SCAF1SRI
(Plasmid
#249044)
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PurposeHis tagged SRI domain of SCAF1 expressed together with His-SUMO, for domain purification (codon optimized sequence of SRI domain)
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Depositing Lab
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Depositing OrganizationUniversity of Copenhagen
Located in Denmark -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHis-SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSRI domain of SCAF1
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SpeciesSynthetic
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2025.12.08.691990 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHisSUMO-SCAF1SRI was a gift from Lea Gregersen (Addgene plasmid # 249044 ; http://n2t.net/addgene:249044 ; RRID:Addgene_249044)