SCAF1-mAID-FLAG degron plasmid
(Plasmid
#249045)
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Purposefor endogenously auxin-degron cassete tagging SCAF1 gene locus in mouse cells (C-terminus insertion)
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Depositing Lab
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Depositing OrganizationUniversity of Copenhagen
Located in Denmark -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC
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Vector typeBacterial Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSCAF1
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SpeciesM. musculus (mouse)
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Entrez GeneScaf1 (a.k.a. SR-A1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2025.12.08.691990 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SCAF1-mAID-FLAG degron plasmid was a gift from Lea Gregersen (Addgene plasmid # 249045 ; http://n2t.net/addgene:249045 ; RRID:Addgene_249045)
