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pmTagBFP2(GGS)-Fb(LAM2)
(Plasmid #249305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249305 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5048
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pmTagBFP2(GGS)-Fb(LAM2)
  • Species
    Synthetic
  • Insert Size (bp)
    1107
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To make pmTagBFP2(GGS)-Fb(LAM2), LAM2 nanobody was amplified from pGEX6P1-mCherry-Nanobody (LaM-2) (Addgene #162276) and then internally fused to mTagBFP2 amplified from pBAD-mTagBFP2 (Addgene #34632) via 2x(Gly)2Ser linkers. Construct was inserted to pcDNA3.1 plasmid using KpnI and EcoRI restriction enzymes.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmTagBFP2(GGS)-Fb(LAM2) was a gift from Vladislav Verkhusha (Addgene plasmid # 249305 ; http://n2t.net/addgene:249305 ; RRID:Addgene_249305)

  • For your References section:

    Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging. Barykina NV, Carey EM, Oliinyk OS, Mendonça-Gomes JM, de Oliveira S, Nimmerjahn A, Verkhusha VV. Nat Methods, 2026 10.1038/s41592-026-03056-3
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