pAJE-30-Mj-nitroY
(Plasmid #249488)

• Purpose: Expresses the Methanocaldococcus jannaschii Tyr tRNA/tRNA synthetase pair engineered for 3-nitroTyr encoding in E. coli on a low copy origin
• Depositing Lab: Ryan Mehl
• Publication: McGee et al Bio Protoc. 2026 Apr 20;16(8):e5674. doi: 10.21769/BioProtoc.5674. eCollection 2026 Apr 20.

Backbone

• Vector backbone: pAJE
• Backbone size w/o insert (bp): 5943
• Total vector size (bp): 6861
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: 3-nitrotyrosine RS (A7)
• Species: M. jannaschii
• Insert Size (bp): 918
• Mutation: Y32H H70T D158H I159A L162R
• Promoter: lpp

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: tRNA (suppresses TAG codons)
• Species: M. jannaschii
• Insert Size (bp): 87
• Promoter: lpp

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3-nitrotyrosine RS (A7) insert is the same found on Plasmid #174079, the difference being the all-compatible pAJE origin.