Efficient and Site-Specific Incorporation of 3-Nitro-Tyrosine Into Recombinant Proteins in Escherichia coli.
McGee SB, Stanisheuski S, Mehl RA, Cooley RB
Bio Protoc. 2026 Apr 20;16(8):e5674. doi: 10.21769/BioProtoc.5674. eCollection 2026 Apr 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249487 | pAJE-30-Ma-nitroY | Expresses the Methanomethylophilus alvus pyrrolysine tRNA/tRNA synthetase pair engineered for 3-nitroTyr encoding in E. coli on a 30-copy pAJE origin, making it compatible with B95 cells. |
| 249488 | pAJE-30-Mj-nitroY | Expresses the Methanocaldococcus jannaschii Tyr tRNA/tRNA synthetase pair engineered for 3-nitroTyr encoding in E. coli on a low copy origin |