Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24959||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)3300
Entrez Genebicc1-b (a.k.a. bicc1, xBic-C, x-Bic-C, Bicaudal-C)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For sense mRNA cut with NotI, transcribe with SP6
For antisense cut with BamHI, transcribe with T7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xBicaudal-C was a gift from Edward De Robertis (Addgene plasmid # 24959 ; http://n2t.net/addgene:24959 ; RRID:Addgene_24959)
For your References section:The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation. Wessely O, De Robertis EM. Development. 2000 May . 127(10):2053-62. PubMed 10769230
Map uploaded by the depositor.