pZac2.1 GfaABC1D HA-NLGN2 TurboBirA PDZ
(Plasmid
#249590)
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PurposeExpress NL2 Turbo BirA fusion in astrocytes
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNlgn2
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SpeciesM. musculus (mouse)
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MutationFusion of TurboBirA, removed PDZ and placed it at the end of the TurboBirA
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Entrez GeneNlgn2 (a.k.a. NL2, NLG2)
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Tags
/ Fusion Proteins
- TurboID (C terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZac2.1 GfaABC1D HA-NLGN2 TurboBirA PDZ was a gift from Cagla Eroglu (Addgene plasmid # 249590 ; http://n2t.net/addgene:249590 ; RRID:Addgene_249590) -
For your References section:
Neuroligin-2 is ubiquitinated by Nedd4l to control developmental astrocyte morphogenesis. Sakers K, Ramirez JJ, Elazar N, Nagendren L, Soderblom E, Eroglu C. J Cell Biol. 2026 Jul 6;225(7):e202512111. doi: 10.1083/jcb.202512111. Epub 2026 Jun 1. 10.1083/jcb.202512111 PubMed 42223381