Neuroligin-2 is ubiquitinated by Nedd4l to control developmental astrocyte morphogenesis.
Sakers K, Ramirez JJ, Elazar N, Nagendren L, Soderblom E, Eroglu C
J Cell Biol. 2026 Jul 6;225(7):e202512111. doi: 10.1083/jcb.202512111. Epub 2026 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249587 | pAAV GfaABC1D HA-NLGN2 | AAV mediated expression of NLGN2 in astrocytes |
| 249588 | pAAV GfaABC1D HA-NLGN2K749R | AAV mediated expression of NLGN2K749R in astrocytes |
| 249589 | PiggyBAC U6 shNedd4l | Knockdown Nedd4l and integrate sequence into the genome |
| 249590 | pZac2.1 GfaABC1D HA-NLGN2 TurboBirA PDZ | Express NL2 Turbo BirA fusion in astrocytes |
| 249591 | pZac2.1 GfaABC1D HA-NLGN1 TurboBirA PDZ | Express NL1 Turbo BirA fusion in astrocytes |
| 249592 | pZac2.1 GfaABC1D HA-NLGN3 TurboBirA PDZ | Express NL3 Turbo BirA fusion in astrocytes |
| 249593 | pZac2.1 hSyn1 HA-NLGN2 TurboBirA PDZ | Express NL2 Turbo BirA fusion in neurons |
| 249594 | pCAG-HA-NL2dPDZ-RM | Express NL2 lacking PDZ binding motif (amino acids 833-836), resistant to shRNA (RM) |
| 249595 | pCAG-HA-NL2dPRM-RM | Express NL2 lacking polyproline region in the intracellular domain (amino acids 798-805), resistant to shRNA (RM) |
| 249596 | pCAG-HA-NL2-GBM-RM | Express NL2 Y770A, resistant to shRNA (RM) |
| 249597 | pCAG-HA-NL2/1dWW-RM IRES mCherry | Express NL2 - NL1 chimera, mutating amino acids 779-782 (PPDY -> AAAA), resistant to shRNA (RM) |
| 249598 | pCAG-HA-NL2/3WW-RM IRES mCherry | Express NL2 - NL3 chimera, mutating amino acids 766-769 (LPDY -> PPDY), resistant to shRNA (RM) |
| 249599 | pCAG-HA-NL2dWWLrg-RM | Express NL2 lacking WW binding motif and flanking region (deletion of amino acids 766-796), resistant to shRNA (RM) |
| 249600 | pCAG-HA-NL2dWW-RM | Express NL2 lacking WW binding motif (mutation of amino acids 767-770 PPDY -> AAAA), resistant to shRNA (RM) |
| 250933 | pX601-Nlgn2-gRNA2-GfaABC1D-mCherry | Nlgn2 gRNA #2 targeting Rat Nlgn2. Also expresses SaCas9 T2a mCherry under the astrocyte promoter gfaABC1D |
| 250934 | pX601-Nlgn2-gRNA3-GfaABC1D-mCherry | Nlgn2 gRNA #3 targeting Rat Nlgn2. Also expresses SaCas9 T2a mCherry under the astrocyte promoter gfaABC1D |