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pCAG-HA-NL2/1dWW-RM IRES mCherry
(Plasmid #249597)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249597 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nlgn2
  • Species
    M. musculus (mouse)
  • Mutation
    Chimeric protein of NL2 extracellular domain, with NL1 intracellular domain, mutation of amino acids 779-782 to Alanines in NL1. Sense mutations to be resistant to NL2 shRNA
  • Entrez Gene
    Nlgn2 (a.k.a. NL2, NLG2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-NL2/1dWW-RM IRES mCherry was a gift from Cagla Eroglu (Addgene plasmid # 249597 ; http://n2t.net/addgene:249597 ; RRID:Addgene_249597)

  • For your References section:

    Neuroligin-2 is ubiquitinated by Nedd4l to control developmental astrocyte morphogenesis. Sakers K, Ramirez JJ, Elazar N, Nagendren L, Soderblom E, Eroglu C. J Cell Biol. 2026 Jul 6;225(7):e202512111. doi: 10.1083/jcb.202512111. Epub 2026 Jun 1. 10.1083/jcb.202512111 PubMed 42223381
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