pX601-Nlgn2-gRNA3-GfaABC1D-mCherry
(Plasmid
#250934)
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PurposeNlgn2 gRNA #3 targeting Rat Nlgn2. Also expresses SaCas9 T2a mCherry under the astrocyte promoter gfaABC1D
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepX601
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX601-Nlgn2-gRNA3-GfaABC1D-mCherry was a gift from Cagla Eroglu (Addgene plasmid # 250934 ; http://n2t.net/addgene:250934 ; RRID:Addgene_250934) -
For your References section:
Neuroligin-2 is ubiquitinated by Nedd4l to control developmental astrocyte morphogenesis. Sakers K, Ramirez JJ, Elazar N, Nagendren L, Soderblom E, Eroglu C. J Cell Biol. 2026 Jul 6;225(7):e202512111. doi: 10.1083/jcb.202512111. Epub 2026 Jun 1. 10.1083/jcb.202512111 PubMed 42223381