pcDNA3 Lunatic Fringe
(Plasmid
#24962)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLunatic Fringe
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SpeciesX. laevis (frog)
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Entrez Genelfng
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A60G amino acid mutation detected in Addgene sequence.
For injection: Cut with XhoI, transcribe with T7 (sense)
For probe: Cut with BamHI, transcribe with SP6 (antisense)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 Lunatic Fringe was a gift from Edward De Robertis (Addgene plasmid # 24962 ; http://n2t.net/addgene:24962 ; RRID:Addgene_24962) -
For your References section:
Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo. Pera EM, Hou S, Strate I, Wessely O, De Robertis EM. Int J Dev Biol. 2005 . 49(7):781-96. 10.1387/ijdb.052003ep PubMed 16172975