pcDNA3 Lunatic Fringe
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24962||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLunatic Fringe
SpeciesX. laevis (frog)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
A60G amino acid mutation detected in Addgene sequence.
For injection: Cut with XhoI, transcribe with T7 (sense)
For probe: Cut with BamHI, transcribe with SP6 (antisense)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 Lunatic Fringe was a gift from Edward De Robertis (Addgene plasmid # 24962 ; http://n2t.net/addgene:24962 ; RRID:Addgene_24962)
For your References section:Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo. Pera EM, Hou S, Strate I, Wessely O, De Robertis EM. Int J Dev Biol. 2005 . 49(7):781-96. 10.1387/ijdb.052003ep PubMed 16172975