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pCS2 xFrzb-HA
(Plasmid #24979)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 24979 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Frzb-1 HA
  • Species
    X. laevis (frog)
  • Insert Size (bp)
  • Tag / Fusion Protein
    • HA tag internal (YPYDVPDYA)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For sense, cut with NotI, transcribe with SP6.

HA tag is cloned between Ser158 and Met159 of Frezzled by PCR. HA tag includes a AatII diagnostic site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xFrzb-HA was a gift from Edward De Robertis (Addgene plasmid # 24979 ; ; RRID:Addgene_24979)
  • For your References section:

    Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer. Leyns L, Bouwmeester T, Kim SH, Piccolo S, De Robertis EM. Cell. 1997 Mar 21. 88(6):747-56. 10.1016/S0092-8674(00)81921-2 PubMed 9118218