pcDNA5 CMV 3xFLAG XBP1 smut (splice mutant)
(Plasmid
#250303)
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PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged XBP1 with intron-4 (26 nt) and splice site mutation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXBP1
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SpeciesH. sapiens (human)
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Entrez GeneXBP1 (a.k.a. TREB-5, TREB5, XBP-1, XBP2)
- Promoter CMV/TO inducible promoter
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Provides hygromycin resistance only when integrated into FlpIn FRT site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 CMV 3xFLAG XBP1 smut (splice mutant) was a gift from Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 250303 ; http://n2t.net/addgene:250303 ; RRID:Addgene_250303)