Thomas Gonatopoulos-Pournatzis Lab: RBM39 Modulates UPR Signaling Through Regulation of IRE1α Alternative Splicing
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250296 | pLCHKO_CRASP_hgInt_XBP1 | Vector for producing lentivirus to generate cell lines expressing intergenic gRNA and XBP1 minigene |
| 250297 | pLCHKO_CRASP_hgERN1_XBP1 | Vector for producing lentivirus to generate cell lines expressing ERN1 targeting gRNA and XBP1 minigene |
| 250298 | PX461 ABE8e SpCas9 ERN1 ex18 5'ss | Vector for inducing 5' splice site mutation (base editing) |
| 250299 | pcDNA5 CMV 3xFLAG ERN1 FL | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged ERN1 full-length |
| 250300 | pcDNA5 CMV 3xFLAG ERN1 ∆exon18 | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged exon-18 skipping ERN1 |
| 250301 | pcDNA5 CMV 3xFLAG XBP1 u (unspliced) | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged XBP1 including intron-4 (26 nt) |
| 250302 | pcDNA5 CMV 3xFLAG XBP1 s (spliced) | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged XBP1 without intron-4 (26 nt) |
| 250303 | pcDNA5 CMV 3xFLAG XBP1 smut (splice mutant) | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of 3xFLAG-tagged XBP1 with intron-4 (26 nt) and splice site mutation |
| 250494 | CRASP-Seq Gene KO v2 empty | Updated and improved library enabling CRASP-Seq experiments by providing a flexible platform for studying splicing regulation |